| 质粒名称: | pGiantin-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000757 |
| 产品用途: | Expresses Giantin-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: |
Giantin, mScarlet3-H
|
| 物种来源: | H.sapiens, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pGiantin-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pGiantin-mScarlet3-H (aka mYongHong)载体质粒基本信息
pGiantin-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pGiantin-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pGiantin-mScarlet3-H (aka mYongHong)质粒载体简介
pGiantin-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pGiantin-mScarlet3-H (aka mYongHong) 5029 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_757_20241014-BAN4_H03_H03_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5029)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5029
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 572..961
/codon_start=1
/label=GOLGB1
/translation="EPQQSFSEAQQQLCNTRQEVNELRKLLEEERDQRVAAENALSVAE
EQIRRLEHSEWDSSRTPIIGSCGTQEQALLIDLTSNSCRRTRSGVGWKRVLRSLCHSRT
RVPLLAAIYFLMIHVLLILCFTGHL"
polyA_signal 1084..1205
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1212..1667)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1694..1798
/gene="bla"
/label=AmpR promoter
promoter 1800..2157
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2008..2143
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2192..2986
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3218..3265
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3594..4182
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 4357..4660
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 4661..4864
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS join(4893..5029,1..550)
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
ORIGIN
1 gagggtgacc aagggtggcc ccctgccctt ctcctgggac atcctgtccc ctcagttcat
61 gtacggctcc agggccttca cgaagcaccc cgccgacatc cccgactact ggaagcagtc
121 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgccgtgtc
181 cgtggcccag gacacctccc tggaggacgg caccctgatc tacaaggtga agctccgcgg
241 caccaacttc cctcctgacg gccccgtaat gcagaagaag acaatgggct gggaagcatc
301 caccgagcgg ttgtaccccg aggacgtcgt gctgaagggc gacattaagc atgccctgcg
361 cctgaaggac ggcggccggt acctggcgga cttcaagacc acctacaggg ccaagaagcc
421 cgtgcagatg cccggcgcct tcaacatcga ccgcaagttg gacatcacat cccacaacga
481 ggactacacc gtggtggaac agtacgaacg ctccgtggcc cgccactcca ccggcggctc
541 cggtggctcc tccggactca gatctcgagg agaaccgcag caaagctttt ctgaagctca
601 gcagcagcta tgcaacacca gacaggaagt gaatgaatta aggaagctgc tggaagaaga
661 acgagaccaa agagtggctg ctgagaatgc tctctctgtg gccgaggagc agatcagacg
721 gttagagcac agtgaatggg actcttcccg gactcctatc attggctcct gtggcactca
781 ggagcaggca ctgttaatag atcttacaag caacagttgt cgaaggaccc ggagtggcgt
841 tggatggaag cgagtcctgc gttcactctg tcattcacgg acccgagtgc cacttctagc
901 agccatctac tttctaatga ttcatgtcct gctcattctg tgttttacgg gccatctata
961 gagcggccgc gactctagat cataatcagc cataccacat ttgtagaggt tttacttgct
1021 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
1081 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
1141 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
1201 tcttaaggcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
1261 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
1321 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
1381 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
1441 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
1501 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
1561 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
1621 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
1681 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
1741 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
1801 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
1861 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
1921 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
1981 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
2041 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
2101 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
2161 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
2221 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
2281 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
2341 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
2401 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
2461 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
2521 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
2581 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
2641 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
2701 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
2761 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
2821 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
2881 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
2941 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
3001 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
3061 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
3121 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
3181 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
3241 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
3301 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
3361 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
3421 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
3481 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
3541 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
3601 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
3661 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
3721 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
3781 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
3841 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
3901 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
3961 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
4021 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
4081 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
4141 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
4201 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
4261 cctgattctg tggataaccg tattaccgcc atgcattagt tattaatagt aatcaattac
4321 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
4381 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
4441 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
4501 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
4561 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
4621 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
4681 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
4741 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
4801 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
4861 agctggttta gtgaaccgtc agatccgcta gcatggatag caccgaggca gtgatcaagg
4921 agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg
4981 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagct
//
